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HistoFast™ - Staining aggregated proteins in tissue sections

BioChromix HistoFast™ Staining kit, amyloid and prion, for determination the presence of aggregated misfolded protein and amyloid in cryosectioned or paraffin embedded tissue sections. HistoFast™ are for "Research and Development Use".

HistoFast™ Staining can be performed together with antibody staining, using the appropriate microscope setup, and in such be complementary to other immunohistochemistry staining procedures. HistoFast™ can give green-blue color to surrounding tissue to facilitate identification and navigation in sections.

HistoFast™ - Amyloid Staining kit
HistoFast™ amyloid staining technology provides a fast, easy, and accurate staining which is highly fluorescent and the section is then easily evaluated with a fluorescence microscope. The amyloid deposits exhibit distinct fluorescence which makes analysis easy and minimize the risk of mistakes. Read more (PDF).

HistoFast™ - Prion Staining kit
HistoFast™ kit for staining of pathogenic prion (PrPSc) deposits in histological tissue sections. Can be used as a complement to antibody immunofluorescence or immunhisto. The kit is based on the AP106p probe and will stain Prion deposits, for example Scrapie, in various tissue types. Read more (PDF).

For inquiries and orders, please go to On-line ordering or contact directly.

HistoFast™ provides for highly specific amyloid staining and the amyloid deposits exhibit strong fluorescence which makes analysis easy and minimize the risk of misinterpretations. De-paraffinized or frozen tissue samples are incubated in buffer for ten minutes, followed by probe incubation for 30 minutes. After a few wash steps the samples are examined by means of fluorescence microscopy.

Patent pending: HistoFast™ is protected by US 11/579,741 including corresponding applications and additional patent pending by BioChromix.


Tissue preparations is done according to standard procedures	BioChromix AP106h	Perform washing and preparation according to protocol	Evaluate the section using a fluorescence microscope

Example: An amyloid plaque evaluated using fluorescence microscopy. Left: The amyloid deposits exhibit a strong red fluorescence (Zeiss filter set 15). Right: The amyloid plaque, clearly seen as orange/red deposits, overlaid on the surrounding tissue, exhibiting green fluorescence, to facilitate identification (Zeiss filter set 15 + filter set 9).